Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple molecular alterations in mouse lung tumors.

F C Re1, G Manenti, M G Borrello

  • 1Division of Experimental Oncology, Istituto Nazionale Tumori, Milan, Italy.

Molecular Carcinogenesis
|January 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bio-Inspired lipid nanovesicles (iNVs) incorporating membrane proteins from healthy tendon stem cells for targeted protein restoration in tendinopathic <i>in vitro</i> model.

Artificial cells, nanomedicine, and biotechnology·2026
Same author

Toxic effects of biodegradable polylactic acid nanoplastics on developing zebrafish (Danio rerio).

Scientific reports·2025
Same author

PLA/PLGA nanocarriers fabricated by microfluidics-assisted nanoprecipitation and loaded with Rhodamine or gold can be efficiently used to track their cellular uptake and distribution.

International journal of pharmaceutics·2024
Same author

Quantitative and qualitative features of acute phase-adverse events following SARS-CoV-2 vaccination in a large sample of people with multiple sclerosis.

Multiple sclerosis and related disorders·2022
Same author

Lipid nano-vesicles for thyroid hormone encapsulation: A comparison between different fabrication technologies, drug loading, and an in vitro delivery to human tendon stem/progenitor cells in 2D and 3D culture.

International journal of pharmaceutics·2022
Same author

Myogenic commitment of human stem cells by myoblasts Co-culture: a static vs. a dynamic approach.

Artificial cells, nanomedicine, and biotechnology·2022

Urethane-induced mouse lung tumors exhibit specific molecular changes, including Ki-ras mutations and altered expression of retinoblastoma (Rb) and growth arrest-specific-3 (gas-3) genes. These alterations suggest key steps in lung carcinogenesis.

Area of Science:

  • Molecular biology
  • Cancer research
  • Genetics

Background:

  • Lung tumors in mice are often induced by urethane.
  • Understanding the molecular basis of urethane-induced lung tumors is crucial for cancer research.

Purpose of the Study:

  • To investigate molecular alterations in urethane-induced mouse lung tumors.
  • To analyze mutations in the Ki-ras gene and expression of specific genes (SP-A, Rb, gas-3, p53, c-myc, TS).

Main Methods:

  • Analysis of 25 mouse lung tumors induced by urethane.
  • Detection of Ki-ras codon 61 mutations.
  • Gene expression analysis using mRNA levels for SP-A, Rb, gas-3, p53, c-myc, and TS.

Main Results:

  • Ki-ras codon 61 mutations were found in 22 of 25 tumors.

Related Experiment Videos

  • Increased SP-A mRNA levels were observed in all tumors.
  • Reduced Rb and gas-3 transcript levels were detected in tumors.
  • c-myc overexpression was observed without gene amplification.
  • p53 mRNA and TS expression showed minimal changes.

    • Conclusions:

    • Urethane-induced lung tumors in mice show recurrent molecular alterations.
    • Ki-ras mutations, decreased Rb and gas-3 expression, and increased c-myc expression are implicated in lung carcinogenesis.
    • These molecular changes may represent distinct steps in tumor development.