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Streptococcus equi subsp. ruminatorum subsp. nov., isolated from mastitis in small ruminants.
Elena Fernández1, Verena Blume1, Patricia Garrido1
1Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Avenida Puerta de Hierro s/n, Madrid 28040, Spain.
International Journal of Systematic and Evolutionary Microbiology
|November 17, 2004
Summary
A novel subspecies of Streptococcus, Streptococcus equi subsp. ruminatorum, was identified in ovine and caprine mastitis cases. This bacterium is distinct from existing Streptococcus equi subspecies based on phenotypic and genetic analyses.
Area of Science:
- Microbiology
- Bacteriology
- Veterinary Science
Background:
- Mastitis in sheep and goats is a significant concern in animal health.
- Accurate identification of causative agents is crucial for effective treatment and control.
Purpose of the Study:
- To characterize and classify six unknown Streptococcus isolates from ovine and caprine mastitis.
- To determine the phylogenetic and taxonomic relationship of these isolates to known species.
Main Methods:
- Phenotypic characterization including cellular morphology and biochemical tests.
- Molecular methods such as 16S rRNA gene sequencing.
- DNA-DNA hybridization, biotyping, and ribotyping for detailed differentiation.
Main Results:
- The isolates were identified as Gram-positive, catalase-negative, chain-forming cocci.
- 16S rRNA gene sequencing placed the isolates within the Streptococcus genus, closely related to Streptococcus equi (98.8% similarity).
- DNA-DNA pairing showed >70% relatedness to S. equi subsp. equi and S. equi subsp. zooepidemicus, but phenotypic and genetic analyses revealed distinct characteristics.
Conclusions:
- The distinct phenotypic and genetic profiles support the classification of these isolates as a new subspecies.
- The novel subspecies is named Streptococcus equi subsp. ruminatorum subsp. nov.
- The type strain is CECT 5772(T).