Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Developing a peptide-based near-infrared molecular probe for protease sensing.

Wellington Pham1, Yongdoo Choi, Ralph Weissleder

  • 1Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 02129, USA.

Bioconjugate Chemistry
|November 18, 2004
PubMed
Summary

Researchers developed a novel peptide-based near-infrared (NIR) fluorescence probe for sensing protease activity in vivo. This probe shows a significant 7-fold increase in fluorescence upon activation by matrix metalloproteinase-7 (MMP-7).

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Intelligent catalase-coated MnO<sub>2</sub> nanoparticles with programmed oxygen supply and glutathione depletion for enhanced photodynamic therapy.

Journal of materials chemistry. B·2026
Same author

Tumor-Responsive Fluorogenic Spray for Image-Guided Cytoreduction of Disseminated Ovarian Tumors.

Bioconjugate chemistry·2025
Same author

Iron-Doxorubicin Hyaluronan Nanogel as an Effective Targeted Chemotherapy with Modulated Cardiotoxicity.

Biomacromolecules·2025
Same author

Hijacking the hyaluronan assisted iron endocytosis to promote the ferroptosis in anticancer photodynamic therapy.

Carbohydrate polymers·2025
Same author

<i>In vivo</i> senescence imaging nanoprobe targets the associated reactive oxygen species.

Nanoscale·2023
Same author

Aptamer-Gemcitabine Conjugates with Enzymatically Cleavable Linker for Targeted Delivery and Intracellular Drug Release in Cancer Cells.

Pharmaceuticals (Basel, Switzerland)·2022

Area of Science:

  • Biomedical Imaging
  • Molecular Probes
  • Fluorescence Spectroscopy

Background:

  • Near-infrared (NIR) molecular probes are crucial for in vivo biomedical imaging.
  • Developing targeted probes for specific enzyme activity is essential for disease diagnosis.

Purpose of the Study:

  • To design and synthesize a novel peptide-based NIR fluorescence probe.
  • To evaluate the probe's ability to sense protease activity, specifically matrix metalloproteinase-7 (MMP-7).

Main Methods:

  • Constructed a probe integrating a NIR fluorescence emitter (Cy5.5) and absorber (NIRQ820) with a protease-selective peptide sequence.
  • Utilized a matrix metalloproteinase-7 (MMP-7) model to assess probe performance.
  • Measured fluorescence signal changes upon enzymatic cleavage.

Related Experiment Videos

Main Results:

  • The designed probe exhibited efficient energy transfer between Cy5.5 and NIRQ820, resulting in a low initial signal.
  • Upon reaction with MMP-7, the probe's fluorescence signal increased sevenfold.
  • The probe demonstrated a high catalytic efficiency with a K(cat)/K(m) of 100,000 M⁻¹s⁻¹.

Conclusions:

  • The developed peptide-based NIR probe effectively senses MMP-7 activity.
  • The synthetic strategy is adaptable for creating other NIR probes for biomedical applications.
  • This probe holds potential for in vivo imaging and diagnostics targeting protease activity.