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Quantitative analysis of amoA mRNA expression as a new biomarker of ammonia oxidation activities in a complex
1Department of Chemical Engineering, Waseda University, Shinjuku-ku, Tokyo, Japan. yoshiteruaoi@toki.waseda.jp
Aims:
To quantitatively analyse the changes to amoA mRNA (ammonia mono-oxygenase encoding mRNA) profiles in response to a change in ammonia oxidation activity in a complex microbial community.
Methods And Results:
The amoA mRNA levels in both a batch-mode incubation and a continuously fed nitrification reactor were determined by real-time reverse transcription-PCR analysis. The amoA mRNA level changed rapidly in response to the change in environmental conditions which affect ammonia oxidation activity.
Conclusion:
An increase in amoA mRNA level can be detected within 1-2 h in response to an initiation of cell activity whereas a decrease in amoA mRNA level is detected within 24 h in response to a cessation of activity.
Significance And Impact Of The Study:
amoA mRNA, which shows sensitive response to ammonia oxidation activity, can be used as a biomarker of ammonia oxidation activity in wastewater treatment processes where many bacterial species exist.
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