Related Experiment Videos
Effector and regulatory T cells derived from the same T cell clone differ in MHC class II-peptide multimer binding.
Esther N M Nolte-'t Hoen1, Maria Grazia Amoroso, Jetty Veenstra
1Department of Infectious Diseases and Immunology, Division of Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
European Journal of Immunology
|November 19, 2004
Summary
Anergic CD4(+) T cells show reduced binding to MHC class II-peptide multimers, a key tool for T cell detection. This diminished binding correlates with the T cell
Area of Science:
- Immunology
- Cellular Biology
- T cell immunology
Background:
- MHC class II-peptide multimers are essential for detecting antigen-specific CD4(+) T cells.
- Hypo-responsive T cells may exhibit reduced multimer binding, impacting their detection and characterization.
Purpose of the Study:
- To investigate the binding capacity of MHC class II-peptide multimers on anergic (hypo-responsive) CD4(+) T cells at the clonal level.
- To explore factors influencing multimer binding and the role of lipid rafts in this interaction.
Main Methods:
- Comparative analysis of MHC class II-peptide multimer binding between anergic and non-anergic CD4(+) T cells.
- Investigation of multimer internalization and localization within T cells.
- Assessment of lipid raft disruption effects on multimer binding.
Main Results:
- Anergic CD4(+) T cells demonstrated significantly reduced MHC class II-peptide multimer binding compared to non-anergic cells.
- Neither temperature, time, nor peptide valency changes normalized binding.
- Multimers showed inefficient internalization and preferential binding to lipid-poor membrane regions.
- Lipid raft disruption decreased dimer binding in non-anergic cells more than in anergic cells.
- The depth of the anergic state correlated with reduced multimer binding.
Conclusions:
- Differential MHC class II-peptide multimer binding exists between regulatory (anergic) and effector T cells with identical T cell receptors (TCRs).
- Reduced multimer binding in anergic T cells is a characteristic feature linked to their regulatory function.
- These findings highlight the importance of considering T cell activation states when using multimer technology.