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Updated: Aug 20, 2026

Methods for Facilitating Microbial Growth on Pulp Mill Waste Streams and Characterization of the Biodegradation Potential of Cultured Microbes
Published on: December 12, 2013
Biodegradation of Red B dye by Bacillus sp. OY1-2
1Stantec Consulting Ltd., 3260 Devon Drive, Windsor, Ontario, Canada N8X 4L4.
Abstract:
Batch tests were employed to investigate the effects of aerobic and anoxic conditions on the biodegradation of Red B dye by Bacillus OY1-2. Results from batch experiments demonstrated anoxic conditions were beneficial for rapid biodegradation of Red B dye in comparison to aerobic conditions. Biodegradation is a major mechanism in the decolorization of Red B dye in comparison with biosorption, which accounted for only 8% of the total decolorization efficiency. Reactors packed with granular activated carbon (GAC) and inoculated with Bacillus OY1-2 were investigated to treat a synthetic wastewater under anoxic conditions. In the absence of cosubstrates, Red B dye was degraded; however, a significant improvement in degradation resulted with the addition of cosubstrates.
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