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Updated: Aug 20, 2026
High-throughput Measurement of Dictyostelium discoideum Macropinocytosis by Flow Cytometry
Published on: September 10, 2018
A database search for double-strand containing RNAs in Dictyostelium discoideum
Stefan Gräf1, Branimira E Borisova, Wolfgang Nellen
1Institut für Physikalische Biologie, Heinrich-Heine-Universität, D-40225 Düsseldorf, Germany.
Abstract:
In eukaryotic cells, double-stranded RNA is degraded to 21mers and triggers RNA interference. Using a pattern description language, we have searched the EMBL database for sequences with the potential to form double strands in cis in Dictyostelium discoideum. No extended inverted repeats were found in mRNAs. However, the antisense direction of some mRNAs encoding regulatory or developmentally regulated proteins showed the ability to form double-stranded regions. In EST archives, we found potential double strands derived from a few genes, but these transcripts are not continuously encoded in the genome. Most likely, they represent hybrid molecules of sense and antisense RNAs.
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