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Updated: Aug 20, 2026
The Multifaceted Benefits of Protein Co-expression in Escherichia coli
Published on: February 5, 2015
[Construction and expression of the eukaryotic coexpression plasmid containing Ag85B gene of Mycobacterium
Shan Jiang1, Dao-yin Zhu, Xu-dong Luo
1Department of Microbiology, Chongqing University of Medical Sciences, Chongqing 400016, China. meimei0075@sina.com.cn
Aim:
To construct an eukaryotic coexpression plasmid containing Mycobacterium tuberculosis (MTB) Ag85B and IL-12 genes.
Methods:
MTB Ag85B gene and IL-12 gene were cloned into pBudCE4.1 which has multiple promoters to construct recombinant plasmid pBud85B-IL12. The recombinant plasmid was transfected into COS-7 cells and the expression of target genes was assessed by RT-PCR and ELISA.
Results:
The expressions of Ag85B and IL-12 could be detected in COS-7 cells.
Conclusion:
The recombinant plasmid pBud85B-IL12 was constructed and expressed successfully, which lays the foundation for further development of DNA vaccine against tuberculosis.
