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Published on: April 29, 2015
[The role of Angelica polysaccharides in inducing effector molecule release by peritoneal macrophages]
Xing-bin Yang1, Qi-bing Mei, Si-yuan Zhou
1Department of Pharmacology, Fourth Military Medical University, Xi'an 710032, China. xbyph@yahoo.com.cn
Aim:
To observe the effect of Angelica polysaccharides on effector molecule production by peritoneal macrophages.
Methods:
Macrophages were isolated from the peritoneal cavity of BALB/c mice and the primary culture was performed. MTT colorimetry and spectrophotometry were used to examine the effects of Angelica polysaccharides on the releases of effector molecules, such as nitric oxide(NO), tumor necrosis factor-alpha(TNF-alpha), and reactive oxygen species(ROS) as well as inducible nitric oxide synthase (iNOS) and lysozyme(LSZ) activity by peritoneal macrophages.
Results:
Angelica polysaccharides could promote the releases of NO, TNF-alpha and ROS from macrophages and improved iNOS and LSZ activities in macrophages. However, Angelica polysaccharides had no direct cytotoxicity to tumor cells, but the cultural supernatant of macrophages cocultured with Angelica polysaccharides could kill L929 cells.
Conclusion:
Angelica polysaccharides can promote the releases of NO, TNF-alpha and ROS by macrophages. Angelica polysaccharides may indirectly play the role of anti-tumors through increased TNF-alpha production by macrophages.
Insights
Angelica polysaccharides boost macrophage production of nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha), and reactive oxygen species (ROS). This suggests potential indirect anti-tumor effects mediated by macrophages.
Area of Science:
- Immunology
- Pharmacology
Context:
- Peritoneal macrophages play a crucial role in immune responses.
- Angelica polysaccharides are natural compounds with potential therapeutic properties.
Purpose:
- To investigate the impact of Angelica polysaccharides on effector molecule production by peritoneal macrophages.
- To assess the influence of Angelica polysaccharides on nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha), and reactive oxygen species (ROS) release.
- To evaluate the effects on inducible nitric oxide synthase (iNOS) and lysozyme (LSZ) activity.
Summary:
- Angelica polysaccharides significantly promoted the release of NO, TNF-alpha, and ROS from macrophages.
- These polysaccharides also enhanced iNOS and LSZ activities in macrophages.
- While not directly cytotoxic to tumor cells, the supernatant from treated macrophages demonstrated cytotoxicity against L929 cells.
Impact:
- Angelica polysaccharides modulate macrophage effector functions, increasing the production of key inflammatory and cytotoxic mediators.
- The findings suggest a potential indirect anti-tumor mechanism for Angelica polysaccharides, primarily through macrophage activation and subsequent TNF-alpha production.
