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Updated: Aug 20, 2026
Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay
Published on: October 30, 2015
[Study on the TCR Vbeta binding sites in the superantigen staphylococcal enterotoxin D]
Ya-fei Li1, Li Zhong, Xi-hua Zhu
1Department of Epidemiology, Third Military Medical University, Chongqing 400038, China. yafeilye@yahoo.com.cn
Aim:
To study TCR Vbeta binding sites of staphylococcal enterotoxin D(SED).
Methods:
Six SED mutants were constructed by site-directed mutagenesis. The activity of promoting T cell proliferation by the mutants was detected by (3)H-TdR incorporation. For the mutants with decreased mitogenic activity, flow cytometry was used detect their MHC-II binding activity and TCR Vbeta specificity.
Results:
Residue N23 played an important role in the interaction of SED with human TCR Vbeta5. Residue H26 was probably a SED binding site to human TCR Vbetas except for TCR Vbeta5, TCR Vbeta8 and TCR Vbeta12.1.
Conclusion:
Residue N23 is a key TCR Vbeta binding site of SED.
