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Macrophage inflammatory protein-2, neutrophil recruitment and bacterial persistence in an experimental mouse model of
Rahul Mittal1, Sanjay Chhibber, Saroj Sharma
1Department of Microbiology, Biomedical Sciences Block, Panjab University, Chandigarh 160014, India.
Abstract:
This study analyzed macrophage inflammatory protein-2 (MIP-2) production and neutrophil recruitment in urinary tract in response to Pseudomonas aeruginosa in an ascending model of urinary tract infection (UTI) in mice. Both planktonic and biofilm cells of P. aeruginosa were used for inducing UTI in mice. MIP-2 levels determined in urine, bladder and kidney showed maximum MIP-2 production 6 h postinfection, which correlated with neutrophil recruitment. Biofilm cells showed significantly more MIP-2 production and neutrophil recruitment. However, no correlation between bacterial numbers and neutrophil recruitment was observed in urine and kidney tissue. The role of MIP-2 and neutrophils in relation to the persistence of P. aeruginosa in the urinary tract of mice is discussed.
Insights
Pseudomonas aeruginosa infection triggers macrophage inflammatory protein-2 (MIP-2) and neutrophil response in mouse urinary tracts. Biofilm cells induced a stronger inflammatory reaction, impacting bacterial persistence.
Area of Science:
- Immunology
- Microbiology
- Urology
Background:
- Urinary tract infections (UTIs) are common, often caused by Pseudomonas aeruginosa.
- Understanding the host immune response is crucial for developing effective treatments.
Purpose of the Study:
- To investigate macrophage inflammatory protein-2 (MIP-2) production and neutrophil recruitment in response to P. aeruginosa UTI in mice.
- To compare the effects of planktonic and biofilm P. aeruginosa cells on the host immune response.
Main Methods:
- An ascending UTI model was established in mice using both planktonic and biofilm P. aeruginosa cells.
- MIP-2 levels were measured in urine, bladder, and kidney tissues.
- Neutrophil recruitment was assessed in the urinary tract.
Main Results:
- MIP-2 production peaked at 6 hours post-infection, correlating with neutrophil recruitment.
- Biofilm cells of P. aeruginosa induced significantly higher MIP-2 production and neutrophil recruitment compared to planktonic cells.
- No direct correlation was observed between bacterial load and neutrophil recruitment in urine and kidney tissues.
Conclusions:
- MIP-2 and neutrophils play a significant role in the host response to P. aeruginosa UTI.
- Biofilm formation by P. aeruginosa exacerbates the inflammatory response.
- Further research is needed to elucidate the role of MIP-2 and neutrophils in bacterial persistence within the urinary tract.
