Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Flexibility and governance in eukaryotic DNA replication.

Etienne Schwob1

  • 1Institute of Molecular Genetics, CNRS UMR5535 and University Montpellier 2, 1919, route de Mende, 34293 Montpellier, France.

Current Opinion in Microbiology
|November 24, 2004
PubMed
Summary

Eukaryotic DNA replication uses epigenetic influences for origin selection, allowing cells to adapt replication dynamics. Conserved factors and kinases initiate replication, with new methods exploring chromosome replication dynamics.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Replication Stress Is an Actionable Genetic Vulnerability in Desmoplastic Small Round Cell Tumors.

Cancer research·2024
Same author

Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae.

Journal of visualized experiments : JoVE·2022
Same author

Measuring S-Phase Duration from Asynchronous Cells Using Dual EdU-BrdU Pulse-Chase Labeling Flow Cytometry.

Genes·2022
Same author

Long-Term Imaging of DNA Damage and Cell Cycle Progression in Budding Yeast Using Spinning Disk Confocal Microscopy.

Methods in molecular biology (Clifton, N.J.)·2017
Same author

Ensa controls S-phase length by modulating Treslin levels.

Nature communications·2017
Same author

Homeostatic control of START through negative feedback between Cln3-Cdk1 and Rim15/Greatwall kinase in budding yeast.

eLife·2017

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Genetics

Background:

  • Eukaryotic DNA replication initiates at numerous, often uncharacterized DNA sequences known as origins.
  • Origin specification in higher eukaryotes is significantly influenced by epigenetic factors, conferring cellular flexibility.
  • While origin selection varies, the machinery and kinases initiating replication are highly conserved across eukaryotes.

Purpose of the Study:

  • To explore the epigenetic basis for eukaryotic DNA replication origin specification.
  • To understand the conserved factors and kinases involved in initiating DNA replication.
  • To investigate the dynamics of chromosome replication using advanced genomic and single-molecule techniques.

Main Methods:

  • Genome-wide approaches to analyze replication dynamics.

Related Experiment Videos

  • Single-molecule techniques for high-resolution observation of replication.
  • Epigenetic analysis of origin regions.
  • Biochemical assays to study replication initiation factors and kinases.

    • Main Results:

    • Epigenetic modifications play a crucial role in determining replication origin usage in higher eukaryotes.
    • A conserved set of trans-acting factors and initiating kinases are essential for replication competence.
    • Emerging genome-wide and single-molecule methods are providing new insights into replication dynamics.

    Conclusions:

    • Epigenetic influence provides flexibility in eukaryotic DNA replication origin selection, enabling cellular adaptation.
    • Conserved molecular machinery ensures reliable replication initiation.
    • Coordinated replication fork progression is vital for multiple chromosomal functions beyond DNA duplication.