Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Covalent capture: a natural complement to self-assembly.

Jeffrey D Hartgerink1

  • 1Rice University, Department of Chemistry and Bioengineering, 6100 Main Street, MS60, Houston, TX 77005, USA. jdh@rice.edu

Current Opinion in Chemical Biology
|November 24, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tumor Implantation Site of Syngeneic Oral Cancer Models Differentially Induces Site-Dependent Local and Systemic Immunosuppression.

Cancers·2026
Same author

Supramolecular Assembly of Collagen-Mimetic Peptide D-Periodic Fibrils and Nanoassemblies.

Biomacromolecules·2026
Same author

A multidomain peptide hydrogel-liposome composite for controlled release of a cyclic dinucleotide in oral cancer.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

Omnidirectional 3D Printing of Anisotropic Nanofibrous Peptide Hydrogels.

bioRxiv : the preprint server for biology·2025
Same author

Nanofibrous supramolecular peptide hydrogels for controlled release of small-molecule drugs and biologics.

Nature nanotechnology·2025
Same author

De Novo Design of Specific Heterotrimeric Collagen-Like Peptides via Genetic Algorithm.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025

Covalent capture stabilizes self-assembled peptides, enhancing their utility. Various cross-linking methods are used to stabilize diverse natural and unnatural peptide structures for characterization.

Area of Science:

  • Biomaterials Science
  • Supramolecular Chemistry
  • Peptide Engineering

Background:

  • Peptide self-assembly forms complex supramolecular structures.
  • Stabilizing these assemblies is crucial for their application and characterization.
  • Existing methods for stabilization are limited.

Purpose of the Study:

  • To explore covalent capture as a method to stabilize self-assembled peptides.
  • To demonstrate the versatility of covalent capture across various peptide systems.
  • To aid in the characterization of stabilized peptide materials.

Main Methods:

  • Utilizing disulfide bond formation for covalent capture.
  • Employing native chemical ligation for peptide stabilization.
  • Applying olefin metathesis and radical capture techniques.

Related Experiment Videos

  • Investigating oxidative lysine cross-linking strategies.

    • Main Results:

    • Successful stabilization of diverse self-assembled peptides via covalent capture.
    • Characterization of stabilized alpha-helical coiled coils and amyloid-like beta-sheet fibers.
    • Demonstrated utility in stabilizing protein mimics like p53 and elastin fragments.
    • Extended to unnatural peptide constructs including nanotubes and micelles.

    Conclusions:

    • Covalent capture significantly enhances the stability and utility of self-assembled peptides.
    • This approach is broadly applicable to natural and unnatural peptide systems.
    • Stabilized peptide materials are amenable to detailed characterization and potential applications.