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Biologically active components of Physostigma venenosum.

Bin Zhao¹, Shabbir M Moochhala, Su-Yin Tham

¹Centre for Biomedical Sciences, Defence Medical and Environmental Research Institute, DSO National Laboratories (DMERI @ DSO), 27 Medical Drive, #09-01, Singapore 117510, Republic of Singapore. zbin@dso.org.sg

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

|November 24, 2004

Summary

This review details analytical methods for determining physostigmine, a potent acetylcholine esterase inhibitor, and its metabolites in biological samples. It covers techniques like HPLC, CE, and RIA published between 1980 and 2003.

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Area of Science:

Pharmacology
Analytical Chemistry

Background:

Physostigmine, an alkaloid from Physostigma venenosum, is a reversible acetylcholine esterase inhibitor.
It increases acetylcholine concentration in both the central and peripheral nervous systems.

Purpose of the Study:

To provide an overview of analytical methodologies for physostigmine and its metabolite determination.
To review methods published between January 1980 and December 2003.

Main Methods:

Review of published literature on physostigmine analysis.
Focus on High-Performance Liquid Chromatography (HPLC) methods.
Inclusion of Capillary Electrophoresis (CE) and Radioimmunoassay (RIA) methods.

Main Results:

HPLC is the most frequently used technique for physostigmine quantification.

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Various sample preparation, chromatographic, and detection methods are employed.

A summary table includes details on sample type, preparation, and analytical parameters.

Conclusions:

Analytical methods are crucial for studying physostigmine's metabolism and effects.
A range of techniques exist for physostigmine determination in biological matrices.