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Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings
Published on: August 19, 2021
Pseudomonas fluorescens infection by bacteriophage PhiS1: the influence of temperature, host growth phase and media
Sanna Sillankorva1, Rosário Oliveira, Maria João Vieira
1Centro de Engenharia Biológica - CEB, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Abstract:
The influence of host growth temperature, phase and media, together with the effect of infection temperature on bacteriophage PhiS1 infection of Pseudomonas fluorescens were examined. The rates of cell lysis and phage release were determined and showed that the efficacy of phage infection was optimal with host cells grown and infected at 26 degrees C. The host physiological state also affected these rates. Infection was dependent on the presence of cell wall proteins with molecular weights of 17.5+/-1 and 99+/-5 kDa.
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