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Identification, distribution pattern of IS231 elements in Bacillus thuringiensis and their phylogenetic analysis
Tianpei Huang1, Jingjing Liu, Fuping Song
1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100094 Beijing, China.
FEMS Microbiology Letters
|November 24, 2004
Summary
IS231 elements are widespread in Bacillus thuringiensis strains, with novel variants and insertion cassettes identified. This research enhances understanding of these mobile genetic elements in B. thuringiensis.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Bacillus thuringiensis is a bacterium with significant agricultural and environmental applications.
- Understanding the mobile genetic elements within B. thuringiensis is crucial for its biological characterization.
Purpose of the Study:
- To investigate the distribution of IS231 elements in Bacillus thuringiensis.
- To identify and characterize novel IS231 elements and associated mobile genetic elements.
Main Methods:
- Polymerase Chain Reaction (PCR) using a specific oligonucleotide primer.
- Cloning of novel IS231 elements, variants, and insertion cassettes.
- BLAST analysis for sequence identity.
- Phylogenetic analysis using neighbor-joining and UPGMA methods.
Main Results:
- IS231 elements were detected in 20 standard strains and 107 of 111 Chinese isolates of B. thuringiensis.
- Three novel IS231 elements (IS231J, IS231O, IS231Q), five variants, and the MICBth4 insertion cassette were identified.
- IS231J showed high sequence identity to a region near the B. thuringiensis vip1Ac gene.
Conclusions:
- IS231 elements are prevalent in B. thuringiensis populations.
- The discovery of novel IS231 elements and their association with other genes provides insights into B. thuringiensis genome dynamics.
- Phylogenetic analysis helps elucidate the evolutionary relationships of IS231 elements.