Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Membrane traffic: controlling membrane fusion by modifying NSF.

Alan Morgan1, Robert D Burgoyne

  • 1The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Crown St., P.O. Box 147, Liverpool L69 3BX, UK. amorgan@liv.ac.uk

Current Biology : CB
|November 24, 2004
PubMed
Summary

The protein NSF, essential for membrane fusion, can be reversibly inactivated by S-nitrosylation and tyrosine phosphorylation. Cells utilize these distinct modifications of NSF to regulate membrane fusion locally.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Functional analysis of epilepsy-associated GABA<sub>A</sub> receptor mutations using Caenorhabditis elegans.

Epilepsia open·2024
Same author

Tissue distribution of cysteine string protein/DNAJC5 in C. elegans analysed by CRISPR/Cas9-mediated tagging of endogenous DNJ-14.

Cell and tissue research·2024
Same author

A Caenorhabditis elegans model of autosomal dominant adult-onset neuronal ceroid lipofuscinosis identifies ethosuximide as a potential therapeutic.

Human molecular genetics·2022
Same author

Lysosome exocytosis is required for mitosis in mammalian cells.

Biochemical and biophysical research communications·2022
Same author

Cysteine string protein alpha accumulates with early pre-synaptic dysfunction in Alzheimer's disease.

Brain communications·2022
Same author

Proteomic analysis of dietary restriction in yeast reveals a role for Hsp26 in replicative lifespan extension.

The Biochemical journal·2021

Area of Science:

  • Molecular biology
  • Cell biology
  • Protein biochemistry

Background:

  • NSF (N-ethylmaleimide-sensitive factor) is a protein crucial for membrane fusion.
  • NSF was identified over 15 years ago for its role in in vitro membrane fusion.
  • Regulation of NSF activity is critical for cellular processes.

Purpose of the Study:

  • To investigate the regulatory mechanisms of NSF.
  • To identify post-translational modifications that affect NSF activity.
  • To understand how different cell types control membrane fusion.

Main Methods:

  • Studying protein inactivation through S-nitrosylation.
  • Analyzing protein inactivation through tyrosine phosphorylation.
  • Comparing regulatory mechanisms across different cell types.

Related Experiment Videos

Main Results:

  • NSF can be reversibly inactivated by S-nitrosylation.
  • NSF can be reversibly inactivated by tyrosine phosphorylation.
  • Distinct cell types employ unique NSF modifications for localized regulation.

Conclusions:

  • S-nitrosylation and tyrosine phosphorylation are key regulatory modifications of NSF.
  • Post-translational modifications provide a mechanism for localized control of membrane fusion.
  • Cell-specific regulation of NSF ensures proper membrane fusion dynamics.