Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ocular surface epithelial and stem cell development.

J Mario Wolosin1, Murat T Budak, M A Murat Akinci

  • 1Department of Ophthalmology, Mount Sinai School of Medicine, New York 10029, USA. jmario.wolosin@mssm.edu

The International Journal of Developmental Biology
|November 24, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitochondrial and microtubule defects in Exfoliation Glaucoma.

Free radical biology & medicine·2025
Same author

Mitochondrial and Microtubule Defects in Exfoliation Glaucoma.

bioRxiv : the preprint server for biology·2024
Same author

A Keratin 12 Expression-Based Analysis of Stem-Precursor Cells and Differentiation in the Limbal-Corneal Epithelium Using Single-Cell RNA-Seq Data.

Biology·2024
Same author

TGFβ1-driven SMAD2/3 phosphorylation and myofibroblast emergence are fully dependent on the TGFβ1 pre-activation of MAPKs and controlled by maternal leucine zipper kinase.

Cellular signalling·2023
Same author

The c-Myc Oncogene Maintains Corneal Epithelial Architecture at Homeostasis, Modulates p63 Expression, and Enhances Proliferation During Tissue Repair.

Investigative ophthalmology & visual science·2022
Same author

Multi-parametric evaluation of autologous cultivated Limbal epithelial cell transplantation outcomes of Limbal stem cell deficiency due to chemical burn.

BMC ophthalmology·2020
Same journal

Spatiotemporal dynamics of lineage-specific epithelial maturation in the developing mouse stomach.

The International journal of developmental biology·2026
Same journal

Dynein axonemal assembly factors (<i>dnaaf</i>) 5 and 9 are expressed in ciliated organs of zebrafish embryos.

The International journal of developmental biology·2026
Same journal

A chloroquine sensitivity gradient induces tissue regeneration and maintenance phenotypes in planarians.

The International journal of developmental biology·2026
Same journal

Sialylated glycoproteins and sialyltransferases involved in mesoderm-derived organ formation during embryogenesis.

The International journal of developmental biology·2026
Same journal

The <i>Hydra</i> FGF family - dispersed across the genome and expressed locally.

The International journal of developmental biology·2026
Same journal

Correction: Inhibition of COX2 impairs angiogenesis and causes vascular defects in developing zebrafish embryos.

The International journal of developmental biology·2025
See all related articles

Ocular surface development involves PAX6+ cells forming limbo-corneal and conjunctival epithelia. Limbal stem cells, identified by ABCG2, are metabolically isolated and lack gap junctions, differentiating early in development.

Area of Science:

  • Developmental biology
  • Ophthalmology
  • Stem cell biology

Background:

  • The ocular surface, comprising limbo-corneal and conjunctival epithelia, originates from PAX6+ head ectodermal cells.
  • These epithelia are stratified, non-keratinizing, and essential for ocular surface integrity.

Purpose of the Study:

  • To describe the phenotypic features and developmental events in the genesis of ocular surface epithelia.
  • To elucidate the origin and characteristics of limbal stem cells and their divergence from conjunctival lineages.

Main Methods:

  • Analysis of PAX6+ cell populations during optic and lens vesicle formation.
  • Identification and isolation of ocular stem cells using ABCG2 expression and Hoechst 33342 exclusion (side population).
  • Tracking of connexin 43 (Cx43) expression to understand cell-cell communication and lineage divergence.

Related Experiment Videos

Main Results:

  • Limbal stem cells, expressing ABCG2, reside in the limbus and are metabolically isolated, lacking gap junctions.
  • Early divergence of limbo-corneal and conjunctival lineages is indicated by differential Cx43 expression.
  • Limbal stem cell phenotype development precedes recognizable anatomical limbal features.

Conclusions:

  • The study details the developmental origins and unique characteristics of ocular surface stem cells.
  • Early molecular cues, including PAX6 gradients and gene regulators like Id and msh, are implicated in ocular surface development and lineage specification.