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Updated: Aug 12, 2026

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
Published on: September 27, 2016
A new approach to revealing point mutations in DNA analyzed by colorimetric detection
Marsel Kabilov1, Dmitrii Pyshnyi, Grigorii Dymshits
1Institute of Chemical Biology and Fundamental Medicine, SD, RAS, Novosibirsk, Russia. kmr@yandex.ru
Abstract:
A new approach to detection of point mutations in an amplified DNA was developed. The approach is based on highly selective ligation (T4 DNA ligase) of a tandem of short oligonucleotides one of which contains the biotin group. The ligation product is formed only when the hybridization complex DNA/tandem is formed and the tandem is perfect. The hybridization complex DNA/(biotinylated ligation product) was separated from the biotinylated component of the tandem by UV-immobilization of the reaction mixture on a nylon membrane. The immobilized hybridization complex was detected colorimetrically by a streptavidin-alkaline phosphatase cojugate with a chromogenic substrate.
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