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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
Published on: May 3, 2018
Molecular cloning and characterization of a novel human protein phosphatase 2C cDNA (PP2C epsilon*)
Feng Jin1, Chaoneng Ji, Lingfeng Liu
1State Key Laboratory of Genetic Engineering, Institute of Genetics, School of life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
Abstract:
We have isolated a novel cDNA from the human fetal brain cDNA library with homology to the Mg2+ -dependent serine/threonine protein phosphatase 2C (PP2C) family. The cDNA is 3055 bp in length, and the predicted coding region encodes a 360-amino-acid protein, which shows 99% identity to the PP2C epsilon from rat and mouse. Then we term it human PP2C epsilon gene. The gene is mapped to chromosome 3q26.1 and contains 4 exons. RT-PCR analysis shows that the PP2C epsilon is widely expressed in human tissues and the expression levels in heart, placenta, lung, liver, kidney, and pancreas are relatively high.
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