Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

VanE-type vancomycin-resistant Enterococcus faecalis clinical isolates from Australia.

Lorena Abadía-Patiño1, Keryn Christiansen, Jan Bell

  • 1Unité des Agents Antibactériens, Institut Pasteur, Paris, France.

Antimicrobial Agents and Chemotherapy
|November 25, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Australian Group on Antimicrobial Resistance (AGAR) Australian Gram-negative Surveillance Outcome Program (GnSOP) Bloodstream Infection Annual Report 2024.

Communicable diseases intelligence (2018)·2025
Same author

Australian Group on Antimicrobial Resistance (AGAR) surveillance outcome programs - bloodstream infections and antimicrobial resistance patterns in Australian children and adolescents, January 2022 - December 2023.

Communicable diseases intelligence (2018)·2025
Same author

Colorectal cancer-associated <i>Streptococcus gallolyticus</i>: a hidden diversity expose.

Journal of bacteriology·2025
Same author

Australian Group on Antimicrobial Resistance surveillance outcome programs - bloodstream infections and antimicrobial resistance patterns from patients less than 18 years of age, January 2020 - December 2021.

Communicable diseases intelligence (2018)·2024
Same author

Global scenario of the RmtE pan-aminoglycoside-resistance mechanism: emergence of the <i>rmtE4</i> gene in South America associated with a hospital-related IncL plasmid.

Microbial genomics·2023
Same author

Detection of <i>Streptococcus gallolyticus</i> and Four Other CRC-Associated Bacteria in Patient Stools Reveals a Potential "Driver" Role for Enterotoxigenic <i>Bacteroides fragilis</i>.

Frontiers in cellular and infection microbiology·2022
Same journal

Genomic diversity and topical antimicrobial resistance in <i>Staphylococcus aureus</i> from clinical and carriage populations in the New York-New Jersey region.

Antimicrobial agents and chemotherapy·2026
Same journal

Characterization of GUA-1, a chromosomally encoded ESBL from <i>Pseudomonas guariconensis-like</i>.

Antimicrobial agents and chemotherapy·2026
Same journal

A monoclonal antibody targeting OXA23 restores carbapenem susceptibility in global epidemic carbapenem-resistant <i>Acinetobacter baumannii</i>.

Antimicrobial agents and chemotherapy·2026
Same journal

Anti-RSV drug screening and inhibition of RSV infection by lapatinib through the IL-17 pathway.

Antimicrobial agents and chemotherapy·2026
Same journal

Methodological considerations for the establishment of epidemiological cutoff values for <i>Sporothrix</i> species using CLSI broth microdilution.

Antimicrobial agents and chemotherapy·2026
Same journal

A community-based One Health approach to the pandemic dynamics of <i>Escherichia coli</i> ST131.

Antimicrobial agents and chemotherapy·2026
See all related articles

Three Australian Enterococcus faecalis isolates with VanE-type resistance were analyzed. Gene expression varied between constitutive and inducible, with cotranscription of vanE operons identified.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Antimicrobial Resistance

Background:

  • Enterococcus faecalis is a significant opportunistic pathogen.
  • Vancomycin resistance in enterococci is a growing public health concern.
  • The VanE type resistance mechanism requires further characterization.

Purpose of the Study:

  • To investigate the molecular basis of VanE-type vancomycin resistance in three Enterococcus faecalis isolates from Australia.
  • To analyze the expression patterns and transcriptional regulation of the vanE operon.

Main Methods:

  • Phenotypic characterization of vancomycin resistance.
  • Genetic analysis including sequencing of vanE operon genes.
  • Gene expression analysis using techniques like RT-PCR or Northern blotting (implied).

Related Experiment Videos

  • Transcription initiation site mapping.

    • Main Results:

    • Three distinct Enterococcus faecalis VanE-type isolates (BM4574, BM4575, BM4576) were identified in Australia.
    • Isolate BM4575 exhibited constitutive vanE gene expression, likely due to a 2-bp deletion in the vanSE gene.
    • Isolates BM4574 and BM4576 displayed inducible vanE gene expression.
    • Transcription analysis indicated that the five genes within the vanE operon are likely cotranscribed from a single promoter located upstream of the vanE gene.

    Conclusions:

    • The study elucidates the differential expression mechanisms of vanE-type resistance in Enterococcus faecalis.
    • A specific genetic mutation (2-bp deletion in vanSE) can lead to constitutive resistance.
    • The vanE operon is transcribed as a single unit, suggesting coordinated regulation of resistance genes.