Related Experiment Video
Updated: Aug 12, 2026

Measurements of Physiological Stress Responses in C. Elegans
Published on: May 21, 2020
[Heat shock induced transcription of hsp90alpha gene on chromatin template]
Zhao-yong Li1, Hui Dai, Jun Yang
1National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Objective:
A CAT reporter plasmid (pBLCAT3alpha1) driven by the promoter of hsp90alpha was in vitro assembled into chromatin to investigate the transcription activity of the reporter gene upon heat shock.
Methods:
A competitive RT-PCR-based technique was used to quantify the promoter activity of hsp90alpha gene on chromatin or naked DNA templates in vitro.
Results:
The in vitro transcription efficiency was first optimized by using different amounts of whole cell extracts from heat shock-treated HeLa cells. In vitro chromatin assembly was carried out with purified components of chromatin assembly associated factors, core histones and CAT reporter gene driven by the promoter of hsp90alpha gene. Results showed that chromatin formation repressed the in vitro transcription of the gene.
Conclusion:
The heat shock induced transcription of hsp90alpha gene on chromatin template is more efficient than that on naked DNA in vitro.
More Related Videos
Related Concept Videos
Chromatin Structure Regulates pre-mRNA Processing
The chromatin structure, especially...
Molecular Chaperones and Protein Folding
The...
Chromatin Immunoprecipitation- ChIP
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
Regulation of the Unfolded Protein Response
Bacterial Protein Maturation
Other Stress Responses in Bacteria

