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Updated: Aug 20, 2026
Isolation and Immortalization of Patient-derived Cell Lines from Muscle Biopsy for Disease Modeling
Published on: January 18, 2015
[Establishment of immortalized cell line BLTR-4 and primary identification of its biological character]
Shan Zheng1, Su-ping Guo, Zu-gen He
1Department of Pathology, Cancer Hospital, CAMS and PUMC, Beijing 100021, China.
Objective:
To establish immortalized cell line from the urothelium of the urinary bladder and identify the characteristics of the cell line.
Methods:
Human papillomavirus 16 (HPV-16) plasmid was used to transfect urothelium of infant urinary bladder in vitro with the help of Fugene-6, and this plasmid contained E6 and E7 genes of HPV-16. We also identified the existence of HPV-16 E6 and E7 genes and the biological characteristics of the cell line by PCR, immunohistochemistry, and the biology identification.
Results:
BLTR-4 cell line, produced from the transfection of HPV-16K plasmid, was a cell line from urothelium with the expression of HPV-16 E6 and E7 genes. It had been cultured more than 70 passages, and the characteristics of growth was similar to the immortalized cell line as reported.
Conclusions:
BLTR-4 cell line is an immortalized cell line from urothelium of the urinary bladder, which contains HPV-16 E6 and E7 genes. BLTR-4 cell line is a good experimental model to investigate the relationship of the infection of high risk HPV and transitional cell carcinoma (TCC) in vitro.
