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A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia
Published on: December 4, 2018
[Expression and DNA binding activity of NF-kappaB in mucosal tissue of chronic rhinosinusitis]
Tong-li Li1, Yuan Li, Ge-hua Zhang
1Department of Otorhinolaryngology Head and Neck Surgery, Third Affiliated Hospital, Sun Yet-sen University, Guangzhou 510630, China.
Objective:
To study the expression and activation of nuclear factor-kappa B (NF-kappaB) in mucosa of rhinosinusitis.
Methods:
The expressions of NF-kappaB p50 and p65 in the mucosa from 18 patients and 10 norms were detected with the method of immunohistochemistry. The activation of DNA-binding proteins which was labelled with 32P-radiolabeled oligonucleotide probe for NF-kappaB was detected with electrophoretic mobility shift assays (EMSA) in mucosa.
Results:
Plasma of epithelial and glandular cells displayed a strongly positive staining reaction to p65, and nucleus displayed a strongly positive staining reaction to p50 (7.8%-52.1%). There was significantly difference (chi2 = 22.917, P < 0.01). The DNA-binding proteins activity of 18 samples from patients with chronic rhinosinusitis (28.14 +/- 16.71) was stronger than that (9.28 +/- 2.84) in normal subjects (t = 4.56, P < 0.05).
Conclusions:
The expression and DNA-binding proteins activity of NF-kappaB was enhanced. It indicated that NF-kappaB was activated in mucosal inflammation of chronic rhinosinusitis.
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