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Updated: Aug 20, 2026

Isolation and Culturing of Primary Murine Adipocytes from Lean and Obese Mice
Published on: January 24, 2025
[Effects of physical activity on adipose tissue cellularity in premenopausal obese women in Bénin]
Pierre Dansou1, Mahoutin Lucrèce Kotin, Anatole Laleye
1Laboratoire Activités physiques et sportives (APS) et motricité, Institut national de la jeunesse, de l'éducation physique et du sport (INJEPS), Université d'Abomey Calavi (UAC), 01 BP 169, Porto-Novo, Bénin. kingpierre1@hotmail.com
Abstract:
The purpose of this article was to quantify the exercise load and investigate the influence of physical exercise on adipose tissue in obese women from Bénin. Twelve subjects participated at the study. Six of the subjects trained three times a week for six months. Each training session lasted forty-five minutes. The remaining six women constituted the control group. Biopsy samples of adipose tissue were taken from the same site in the gluteal iliac region at the end of six months and analyzed by histochemistry and electron microscopy. The results showed that all subjects suffered from severe central obesity; lipid content and number of adipocytes were higher among the sedentary woman than among those who exercised. Intense exercise in the latter used more lipids than carbohydrates. We conclude that regular, supervised physical exercise reduces lipid levels and thus induces weight loss.
