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Updated: Aug 20, 2026
Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
[Evaluation of an automated method for measuring low-density lipoprotein cholesterol]
C Désidéri-Vaillant1, S Bouvier, B Suignard
1Laboratoire de biochimie, toxicologie et pharmacologie cliniques, Hôpital d'instruction des armées Clermont Tonnerre, Brest. biochimie@hopital-armees-brest.fr
Abstract:
We evaluated an automated method for measuring low-density lipoprotein cholesterol (LDL-cholesterol) with Integra 800 (Roche Diagnostics). This method uses detergents which show selectivity for the different lipoproteins. Our evaluation has shown a within run imprecision of 2% and a between-assay imprecision of 1.2%. This method was not perturbed by haemoglobin, bilirubin or lipids. However, the reagents remain expensive: we will use it when calculation or interpretation of lipid results are not possible.
