Clinical laboratory advances in the detection of rabies virus

Rabies is one of the most feared zoonotic diseases in the world. All warm-blooded animals are susceptible to infection by the virus, but the main vectors of human infection are dogs and cats. Development of rabies can be prevented by postexposure vaccination, and with a few exceptions, the exact time and source of human infection is usually known. However, the effective use of postexposure vaccination depends on the rapid and accurate detection of rabies virus in specimens obtained from the source of human infection. This paper provides an overview on developments on laboratory methods for the early detection of rabies virus. In most laboratories, the fluorescent antibody test (FAT) is used as the most important primary test, with the rabies tissue culture infection test (RTCIT) or the mouse inoculation test (MIT) being used as confirmatory backup procedures. However, other methods for the detection of antigens, such as rapid rabies-specific enzyme-linked immunosorbent assay (rapid-ELISA) and the detection of viral nucleic acids by reverse transcription polymerase chain reaction (RT-PCR) are increasingly being used for diagnosis and, in combination with nucleotide sequencing, for epidemiological investigations.