Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bax-induced cell death in Candida albicans.

Kris De Smet1, Ines Eberhardt, Rieka Reekmans

  • 1Fundamental and Applied Molecular Biology, Department for Molecular Biomedical Research, Ghent University and VIB, Technologiepark 927, B-9052 Gent, Belgium.

Yeast (Chichester, England)
|November 27, 2004
PubMed
Summary

The pro-apoptotic protein Bax induces cell death in the pathogenic yeast Candida albicans when its gene is expressed. Bax expression also causes mitochondria to cluster near the nucleus.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Off-target glycans encountered along the synthetic biology route toward humanized N-glycans in Pichia pastoris.

Biotechnology and bioengineering·2020
Same author

Pre-clinical Evaluation of a New Antimicrobial Enzyme for the Control of Wound Bioburden.

Wounds : a compendium of clinical research and practice·2015
Same author

The low global burden of trichinellosis: evidence and implications.

International journal for parasitology·2014
Same author

Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate.

Microbial cell factories·2010
Same author

Alteration of N-glycome in diethylnitrosamine-induced hepatocellular carcinoma mice: a non-invasive monitoring tool for liver cancer.

Liver international : official journal of the International Association for the Study of the Liver·2010
Same author

Altered serum N-glycomics in chronic hepatitis B patients.

Liver international : official journal of the International Association for the Study of the Liver·2009

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Mycology

Background:

  • Bax is a pro-apoptotic protein regulating programmed cell death in mammals.
  • Heterologous expression of Bax induces cell death in various yeast species.

Purpose of the Study:

  • To investigate the effects of Bax expression in the pathogenic yeast Candida albicans.
  • To determine if Bax induces cell death and affects mitochondrial localization in C. albicans.

Main Methods:

  • Codon-optimized synthetic BAX gene expression in C. albicans.
  • Fusion of Bax with enhanced green fluorescent protein (EGFP) for stability and visualization.
  • Mitochondrial localization studies using fluorescence microscopy.

Main Results:

Related Experiment Videos

  • Expression of codon-optimized BAX gene inhibited growth and induced cell death in C. albicans.
  • Bax-EGFP fusion protein showed increased cell death-inducing activity.
  • Expressed Bax co-localized with mitochondria and caused them to cluster in the perinuclear region.

Conclusions:

  • Bax expression can induce cell death in the pathogenic yeast C. albicans.
  • Bax affects mitochondrial distribution in yeast, a novel finding.
  • Bax represents a potential target for antifungal strategies against C. albicans.