Related Experiment Video
Updated: Jul 4, 2026

Expression, Solubilization, and Purification of Eukaryotic Borate Transporters
Published on: March 7, 2019
Molecular cloning and characterization of a human urate transporter (hURAT1) gene promoter
Tao Li1, Jacob R Walsh, Fayez K Ghishan
1Department of Pediatrics, Steele Memorial Children's Research Center, University of Arizona Health Sciences Center, Room 3325, 1501 North Campbell Avenue, Tucson, AZ 85724, USA.
Abstract:
We report the novel cloning and preliminary characterization of a human urate transporter (hURAT1) gene promoter. The transcription initiation site was mapped to a base 337 bp upstream of the ATG start codon by primer extension and 5'-RACE. The minimal functional promoter region is within 253 bp when the promoter/luciferase constructs were transfected into OK cells. The sex hormone testosterone significantly increases promoter activity, suggesting that hormonal regulation of hURAT1 is the root cause of observed differences in urate levels between males and females.

