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Subnucleolar distribution and organization of Vicia faba L. rDNA in situ
Hong Long1, Haijing Sun, Xianlu Zeng
1Institute of Genetics and Cell Biology, Northeast Normal University, Changchun 130024, China.
Abstract:
The distribution and organization of nucleolar DNA in Vicia faba L. was analyzed by specific cytochemical staining using NAMA-Ur. The results showed that nucleolar DNA was distributed in the FCs and at the FC/DFC junctions. Statistical analysis showed that the rRNA genes occupied about one-third of the total dense fibrillar component region. The rDNA was condensed in some regions and uncondensed in others. Nucleolus-associated chromatin extended from outside the nucleolus to the periphery of the FCs via nucleolar channels, suggesting a possible origin for nucleolar DNA.
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