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Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
Published on: January 22, 2018
Internal transcribed spacers (ITS) of Trypanosoma rangeli ribosomal DNA (rDNA): a useful marker for inter-specific
I T Beltrame-Botelho1, D Gaspar-Silva, M Steindel
1Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Catarina, Caixa Postal 476, 88040-900, Florianópolis, SC, Brazil.
Abstract:
The internal transcribed spacers (ITS) flanking the 5.8S subunit of the ribosomal RNA genes (rDNA) of Trypanosoma rangeli strains isolated from distinct geographical regions and hosts were studied. The results revealed the sequence variability of the ITS spacers showing the presence of microsatellite repeats and single nucleotide polymorphisms (SNP), which were also observed within the 5.8S rDNA sequence. ITS-2 spacer was the most phylogenetically informative region due the presence of a higher number of parsimonious sites in both inter- and intra-specific analysis. Sequence analysis of both ITS spacers plus the 5.8S rDNA of T. rangeli strains allowed a clear inter-specific differentiation from Trypanosoma cruzi strains representative of the parasite zymodemes.

