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Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
Published on: March 31, 2017
A simple and economic preservation method for genomic bacterial DNA from clinically significant pathogens
John E Moore1, Jiru Xu, B Cherie Millar
1Northern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Lisburn Road, Belfast BT9 7AD, Northern Ireland, United Kingdom. jemoore@niphl.dnet.co.uk
Journal of Microbiological Methods
|November 30, 2004
Summary
Dried bacterial cultures on blood agar provide a simple method for DNA storage. This technique avoids refrigeration and prior DNA extraction, proving useful for transporting microbial DNA globally.
Area of Science:
- Microbiology
- Molecular Biology
- Genomics
Background:
- Preserving bacterial cultures for DNA extraction is crucial for research and diagnostics.
- Current methods often require specialized equipment like refrigerators or freezers, limiting accessibility.
- Pre-storage DNA extraction can be a barrier in resource-limited settings.
Purpose of the Study:
- To evaluate a novel method for storing bacterial genomic DNA.
- To assess the feasibility of room-temperature storage of dried bacterial cultures.
- To determine the suitability of this method for DNA amplification via PCR.
Main Methods:
- Bacterial cultures were dried to completion on Columbia agar base with defibrinated horse blood.
- Cultures were stored at room temperature for 6 months.
- Microbial DNA was subsequently extracted and amplified using the polymerase chain reaction (PCR).
Main Results:
- Successful DNA extraction and PCR amplification were achieved from cultures stored for 6 months at room temperature.
- The method eliminated the need for pre-storage DNA extraction.
- The technique did not require refrigeration or freezing.
Conclusions:
- Drying bacterial cultures on blood agar is a viable and simple method for long-term DNA storage.
- This technique offers advantages over traditional methods, particularly in resource-limited environments.
- It facilitates the transportation and storage of bacterial genomic DNA, even from nonviable cells.
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