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Assembly and budding of influenza virus
Debi P Nayak1, Eric Ka-Wai Hui, Subrata Barman
1Department of Microbiology, Immunology and Molecular Genetics, Jonsson Comprehensive Cancer Center, Molecular Biology Institute, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA. dnayak@ucla.edu
Virus Research
|November 30, 2004
Summary
Influenza virus assembly involves viral envelope proteins, matrix protein (M1), and ribonucleocapsids (vRNPs) concentrating at the plasma membrane. Host factors and viral proteins like M1 are crucial for virus budding and release, offering potential drug targets.
Area of Science:
- Virology
- Cell Biology
- Biochemistry
Background:
- Influenza viruses cause acute febrile respiratory illness.
- Virus particles consist of envelope, matrix protein (M1), and ribonucleocapsid (vRNP).
- Assembly occurs at the apical plasma membrane of polarized epithelial cells.
Purpose of the Study:
- To elucidate the mechanisms of influenza virus morphogenesis and budding.
- To identify viral and host factors involved in virus particle formation and release.
Main Methods:
- Analysis of viral component transport and interactions.
- Investigation of lipid raft involvement in viral protein sorting.
- Examination of matrix protein (M1) and ribonucleocapsid (vRNP) trafficking.
Main Results:
- Viral glycoproteins (HA, NA) use lipid rafts for apical sorting.
- Matrix protein (M1) bridges the viral envelope and vRNP.
- Bud formation involves membrane bending and concentration of viral components.
- Neuraminidase (NA) is essential for virus release from cell surfaces.
Conclusions:
- Influenza virus assembly requires coordinated action of viral and host factors.
- Matrix protein (M1) and lipid rafts play critical roles in morphogenesis.
- Understanding these processes can inform the development of antiviral therapies targeting viral budding.