Related Experiment Video
Updated: Jun 17, 2026
An Improved Method for the Preparation of Type I Collagen From Skin
Published on: January 22, 2014
Effect of all trans-retinoic acid on the secretion of IV collagenase
1Department of General Surgery, First Affiliated Hospital, Dalian Medical University, Dalian 116011, China. wilmawf@sina.com
Background:
Invasion and metastasis cause death of patients with liver cancer. Increased expression of matrix metalloproteinases (MMPs) is closely associated with tumor progression. Type IV collagen is the main structure protein of basilar membrane which is a natural barrier for inhibiting the metastasis of liver cancer cells. In this experiment, we used all trans-retinoic acid (ATRA) to inhibit collagenase type IV in order to protect the type IV collagen and basilar membrane, further to suppress the metastasis of hepatocellular carcinomas.
Methods:
By the use of cell culture and experimental animal models, the influence of all trans-retinoic acid (ATRA) on the invasion of Hca-F liver cancer cells was studied in terms of adhesion capacity to artificial basilar membrane and production of collagenase type IV.
Result:
ATRA could inhibit the adhesion capacity and collagenase IV secretion of Hca-F cells in a dose-dependent manner.
Conclusion:
ATRA can exert multiple effects on the invasion of Hca-F cells.
