Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Determination of the modulation transfer function for a time-gated fluorescence imaging system.

Sarah Gundy1, Wil Van der Putten, Andy Shearer

  • 1National University of Ireland, Galway, National Centre for Biomedical Engineering Science, Galway, Republic of Ireland. sarah.gundy@nuigalway.ie

Journal of Biomedical Optics
|December 1, 2004
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Real-time monitoring of protein-liposome interaction kinetics using absorption, polarized intrinsic emission, and scattering (APIES): insights into protein corona formation.

Nanoscale·2026
Same author

Using Rayleigh-Mie scattered light and polarised multidimensional fluorescence emission in combination for protein quantification in a model clarified bioreactor harvest.

Analytica chimica acta·2025
Same author

Nano- and meso-scale aggregation of poly(N-isopropylacrylamide) below the lower critical solution temperature: A wide-angle dynamic light scattering study.

Journal of colloid and interface science·2025
Same author

Monitoring small-scale bioreactor studies for media development using polarized total synchronous fluorescence spectroscopy (pTSFS) and synchronous light scattering (SyLS).

Journal of biotechnology·2024
Same author

Size exclusion chromatography for screening yeastolate used in cell culture media.

Journal of biotechnology·2023
Same author

Analyzing protein conjugation reactions for antibody-drug conjugate synthesis using polarized excitation emission matrix spectroscopy.

Biotechnology and bioengineering·2022

This study introduces a time-gated fluorescence imaging system for enhanced cancer detection. The novel system improves contrast by utilizing fluorescence lifetimes, enabling clearer visualization of tumors.

Area of Science:

  • Biomedical Optics
  • Medical Imaging
  • Cancer Diagnostics

Background:

  • Current steady-state fluorescence detection methods for cancer exhibit poor contrast due to tumor fluorescence and background autofluorescence.
  • Time-resolved techniques offer improved contrast by differentiating fluorescence lifetimes between autofluorescence and exogenous photosensitizers in tumors.

Purpose of the Study:

  • To develop and characterize a time-gated fluorescence imaging system for enhanced cancer detection.
  • To evaluate the system's ability to improve contrast and resolution in fluorescence imaging.

Main Methods:

  • Constructed an imaging system utilizing a fast-gated (200-ps) charge-coupled device (CCD) camera and a pulsed 635-nm laser diode.
  • Employed an extended knife-edge technique to measure the system's modulation transfer function (MTF).

Related Experiment Videos

  • Imaged the photosensitizer chloroaluminum phthalocyanine tetrasulfonate (AlPcTS) in a polymethyl methacrylate (PMMA) target.

    • Main Results:

    • The system demonstrated the capability to resolve 1 mm fluorescence signals of 10(-4) M AlPcTS.
    • Characterized the system's contrast transfer using MTF measurements.
    • Validated the use of AlPcTS due to its favorable absorption, fluorescence yield, and lifetime (approx. 7.5 ns).

    Conclusions:

    • The developed time-gated fluorescence imaging system shows promise for improved cancer detection.
    • Utilizing fluorescence lifetimes is a viable strategy for enhancing contrast in biomedical imaging.
    • This technology contributes to the advancement of fluorescence-based diagnostic tools.