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Plasmid analysis in PPNG strains isolated in Italy
G Manicardi1, P Aloisi, R Neglia
1Cattedra di Microbiologia, Università degli Studi di Modena, Italy.
Microbiologica
|January 1, 1992
Summary
Penicillin-resistant Neisseria gonorrhoeae (PPNG) strains are uncommon in Italy. This study details the plasmid profiles of two PPNG strains, revealing distinct plasmid sizes and types.
Area of Science:
- Microbiology
- Genetics
Background:
- Penicillin-resistant Neisseria gonorrhoeae (PPNG) strains are a global health concern.
- Reports of PPNG strains in Italy are infrequent.
Purpose of the Study:
- To characterize the plasmidial features of two PPNG strains identified in Italy.
- To analyze the genetic elements associated with penicillin resistance in these isolates.
Main Methods:
- Plasmid DNA was isolated from the two PPNG strains.
- Plasmid DNA was transferred to Escherichia coli via transformation (for "Africa" plasmid) and conjugation (for "Asia" plasmid).
- Restriction endonuclease analysis was performed on the transferred plasmids.
Main Results:
- One PPNG strain harbored plasmids of 3.2 and 2.6 Mdal.
- The second PPNG strain contained plasmids of 24.5, 4.5, and 2.6 Mdal.
- Restriction analysis provided insights into the specific plasmid types present.
Conclusions:
- The study provides the first detailed plasmid characterization of PPNG strains in Italy.
- The findings contribute to understanding the genetic basis of penicillin resistance in local Neisseria gonorrhoeae populations.