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Plasmid analysis in PPNG strains isolated in Italy
G Manicardi1, P Aloisi, R Neglia
1Cattedra di Microbiologia, Università degli Studi di Modena, Italy.
Microbiologica
|January 1, 1992
Abstract:
Italian reports of PPNG strains are rare. In this paper we report the plasmidial characteristics of two strains: one harbouring 3.2 and 2.6 Mdal plasmids and the other 24.5, 4.5 and 2.6 Mdal plasmids. Restriction analysis was carried out on plasmids transferred to E. coli by transformation for "Africa" and conjugation for "Asia" plasmid.
Insights
Penicillin-resistant Neisseria gonorrhoeae (PPNG) strains are uncommon in Italy. This study details the plasmid profiles of two PPNG strains, revealing distinct plasmid sizes and types.
Area of Science:
- Microbiology
- Genetics
Background:
- Penicillin-resistant Neisseria gonorrhoeae (PPNG) strains are a global health concern.
- Reports of PPNG strains in Italy are infrequent.
Purpose of the Study:
- To characterize the plasmidial features of two PPNG strains identified in Italy.
- To analyze the genetic elements associated with penicillin resistance in these isolates.
Main Methods:
- Plasmid DNA was isolated from the two PPNG strains.
- Plasmid DNA was transferred to Escherichia coli via transformation (for "Africa" plasmid) and conjugation (for "Asia" plasmid).
- Restriction endonuclease analysis was performed on the transferred plasmids.
Main Results:
- One PPNG strain harbored plasmids of 3.2 and 2.6 Mdal.
- The second PPNG strain contained plasmids of 24.5, 4.5, and 2.6 Mdal.
- Restriction analysis provided insights into the specific plasmid types present.
Conclusions:
- The study provides the first detailed plasmid characterization of PPNG strains in Italy.
- The findings contribute to understanding the genetic basis of penicillin resistance in local Neisseria gonorrhoeae populations.