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Related Experiment Videos

[Evaluation of renal damage using urinary ATP analysis].

Yuki Uehara1, Mitsuru Yanai, Kazunari Kumasaka

  • 1Department of Laboratory Medicine, Nihon University School of Medicine, Tokyo, Japan.

Nihon Jinzo Gakkai Shi
|December 2, 2004
PubMed
Summary

Urinary adenosine triphosphate (ATP) analysis offers insights into urinary tract infections and drug-induced kidney damage. Establishing a reference range of 1.77 x 10(-10) to 7.70 x 10(-9) M is crucial, with careful consideration for samples containing red blood cells (RBCs) and white blood cells (WBCs).

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Area of Science:

  • Biochemistry
  • Clinical Chemistry
  • Urology

Context:

  • Urinary adenosine triphosphate (ATP) concentration analysis is recognized for its diagnostic utility in identifying urinary tract infections and drug-induced renal damage.
  • The firefly luciferin-luciferase assay is a sensitive method for quantifying urinary ATP.
  • Establishing reliable reference values and understanding pre-analytical factors are essential for accurate clinical interpretation.

Purpose:

  • To determine the reference value range for urinary free ATP concentration in healthy individuals.
  • To evaluate the impact of urine sediments, specifically red blood cells (RBCs) and white blood cells (WBCs), on ATP measurements.
  • To assess the effects of different storage conditions (refrigeration, freezing) and sample preparation methods (centrifugation, filtration) on urinary ATP stability.

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Summary:

  • A reference range for urinary free ATP was established as 1.77 x 10(-10) to 7.70 x 10(-9) M based on samples from 63 outpatients without renal disease.
  • ATP concentrations were significantly reduced after centrifugation and filtration, particularly in samples with higher RBC and WBC counts, suggesting potential for artificially elevated readings without sample preparation.
  • Urinary ATP showed stability with refrigeration but not with freezing, highlighting the importance of appropriate sample handling.

Impact:

  • Provides a validated reference range for urinary free ATP, aiding in the clinical assessment of renal health and urinary tract infections.
  • Highlights the critical need to account for urine sediment content (RBCs, WBCs) and employ appropriate sample preparation techniques to avoid inaccurate ATP quantification.
  • Informs best practices for urine sample collection, storage, and processing to ensure the reliability of urinary ATP measurements in diagnostic settings.