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Published on: December 8, 2023
Cloning, expression and purification of SmpB from Mycobacterium tuberculosis
L Kovács1, Klára Megyeri, Anna Juhász
1Department of Medical Microbiology and Immunobiology, University of Szeged, PO Box 427, H-6701 Szeged, Szeged, Hungary.
Acta Microbiologica Et Immunologica Hungarica
|December 2, 2004
Abstract:
SmpB, a small tmRNA binding protein, is essential for trans-translation. 6His and FLAG tagged SmpB was cloned from Mycobacterium tuberculosis H37Rv. It was expressed in Escherichia coli using the T7 promoter-polymerase system. Anti-FLAG M2 agarose was used for its purification. Mycobacterial SmpB copurifies with other proteins. We identified elongation factor EF-Tu in the purified SmpB preparations.
