Related Experiment Video
Updated: Aug 20, 2026

Functional Assessment of Intestinal Permeability and Neutrophil Transepithelial Migration in Mice using a Standardized Intestinal Loop Model
Published on: February 11, 2021
Leptin fluctuates in intestinal ischemia-reperfusion injury as inflammatory cytokine
Ji Lin1, Guang-Tao Yan, Lu-Huan Wang
1Research Laboratory of Biochemistry, Basic Medical Institute, General Hospital of PLA, 28 Fuxing Road, Beijing 100853, PR China.
Abstract:
As leptin is an active mediator mainly secreted by adipose tissue and is closely related with energy metabolism, we evaluate both the changes of leptin levels in serum and adipose tissue with a concise radioimmunoassay and the changes of leptin mRNA expression in adipose tissue with RT-PCR, during the severe metabolic impediment in rat intestinal ischemia-reperfusion (I/R) injury. Results show that not only leptin levels in serum and adipose tissue but also its mRNA expression in adipose tissue undergo a fluctuation according to different injury times. Therefore, we conclude that leptin has a time-dependent response to acute inflammatory stimuli and acts as an anti-inflammatory cytokine.
Related Concept Videos
Acute Inflammation III: Local and Systemic Effects
Inflammatory Bowel Disease II: Ulcerative Colitis
Amebiasis
Acute Inflammation II: Cellular Phase
Inflammatory Bowel Disease III: Crohn's Disease

