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Hematopoietic cells regulate the angiogenic switch during tumorigenesis
Rika Okamoto1, Masaya Ueno, Yoshihiro Yamada
1Department of Stem Cell Biology, Cancer Research Institute of Kanazawa University, 13-1 Takara-machi, Kanazawa 920-0934, Japan.
Blood
|December 2, 2004
Summary
Hematopoietic cells (HCs) are crucial for tumor blood vessel formation. Their specific localization and function in tumor angiogenesis vary by cancer type, impacting treatment strategies.
Area of Science:
- Oncology
- Hematology
- Vascular Biology
Background:
- Hematopoietic cells (HCs) contribute to blood vessel formation through secreted factors.
- Tumorigenesis involves complex interactions between cancer cells and the tumor microenvironment.
Purpose of the Study:
- To investigate the role and localization of HCs in tumor angiogenesis.
- To understand how HC behavior differs between colon cancer (colon26) and prostate cancer (PC3) models.
- To determine the impact of modulating HCs on tumor angiogenesis.
Main Methods:
- Injected colon26 or PC3 cells into mice to establish tumors.
- Analyzed HC localization within developing tumors using microscopy.
- Administered anti-c-Kit neutralizing antibody to induce bone marrow suppression.
- Assessed the effects of bone marrow suppression on tumor angiogenesis.
Main Results:
- HCs localized within the inner tumor mass for both colon26 and PC3 models.
- Mature HCs infiltrated colon26 tumors early, preceding capillary formation.
- c-Kit+ hematopoietic stem/progenitor cells accumulated at the periphery of PC3 tumors.
- Bone marrow suppression differentially affected angiogenesis: inhibiting initiation in colon26 and vessel caliber in PC3 tumors.
Conclusions:
- HCs play a significant role in tumor angiogenesis.
- HC localization and function are tumor cell-type dependent.
- Targeting HCs may offer distinct therapeutic strategies for different cancers.