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Effect of piroxicam on matrix metalloproteinase 2 and apoptosis

A Mirshafiey1, F Vaezzadeh, M R Khorramizadeh

  • 1Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. a_mirshafiey@yahoo.com

International Journal of Tissue Reactions
|December 3, 2004
PubMed

Insights

Piroxicam, a nonsteroidal anti-inflammatory drug (NSAID), effectively reduces matrix metalloproteinase 2 (MMP-2) activity and promotes apoptosis in fibrosarcoma cells. It demonstrated superior tolerability compared to diclofenac and dexamethasone.

Area of Science:

  • Pharmacology
  • Cancer Biology
  • Cellular Biology

Background:

  • Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used for their anti-inflammatory properties.
  • Matrix metalloproteinase 2 (MMP-2) plays a role in tumor invasion and metastasis.
  • Apoptosis, or programmed cell death, is a critical mechanism for controlling cell proliferation.

Purpose of the Study:

  • To investigate the effects of piroxicam on apoptosis and MMP-2 activity in fibrosarcoma cells.
  • To compare the efficacy and tolerability of piroxicam with diclofenac and dexamethasone.
  • To evaluate piroxicam's potential as an anti-cancer agent.

Main Methods:

  • Utilized the WEHI-164 fibrosarcoma cell line for in vitro experiments.
  • Assessed MMP-2 activity using zymography.
  • Quantified apoptosis via the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay.
  • Administered drugs at concentrations ranging from 10-200 microg/ml with 2-fold serial dilutions.

Main Results:

  • Piroxicam significantly diminished MMP-2 activity in fibrosarcoma cells.
  • Piroxicam treatment led to a notable induction of apoptosis.
  • Piroxicam exhibited higher tolerability compared to diclofenac and dexamethasone.

Conclusions:

  • Piroxicam demonstrates the capacity to induce apoptosis and suppress MMP-2 activity.
  • These findings suggest piroxicam's potential therapeutic value in managing fibrosarcoma.
  • Piroxicam presents a favorable tolerability profile for potential clinical application.

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