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Updated: Aug 20, 2026
Induction of Diffuse Axonal Brain Injury in Rats Based on Rotational Acceleration
Published on: May 9, 2020
[A study on expression of hsp70 mRNA in the early stage of diffuse brain injury in rats]
Ye Wang1, Hong-fu Pan, Kui-ming Zhang
1Department of Forensic Pathology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Objective:
To evaluate the changes of hsp70 mRNA in neuron and glia of rat suffering early diffuse brain injury.
Methods:
SD rats were used in the replication of the animal pattern of diffuse brain injury. In situ hybridization technique and image analysis technique were applied in detecting the hsp70 mRNA in the rat's cerebral cortex, thalamus and brain stem.
Results:
The signal intensity of hsp70 mRNA could be detected in 5 minutes after brain injury; subsequently, it attained to the summit at 6 hours and declined at 12 hours after brain injury.
Conclusion:
The above data suggest that hsp70 mRNA is useful for the diagnosis of early diffuse brain injury and for distinguishing the postmortem injury from antemortem injury.
