Related Experiment Video
Updated: Aug 20, 2026
Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA
Published on: December 15, 2023
[Applying competitive polymerase chain reaction to the detection of hepatitis B virus DNA]
Ling Wang1, Peng Yang, Shuang-qing Li
1Lab of Molecule Genetics of Eye Disease, West China Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To reduce the rate of accidental false negative result in the HBV DNA PCR test on clinical serum samples.
Methods:
A competitive polymerase chain reaction (C-PCR) was used to decrease the false negative ratio. In the C-PCR, a constructed inner control DNA was added for co-amplification with the HBV target DNA.
Results:
In a 20 microl C-PCR system, about 60 to 200 copies of inner control DNA could give apparent co-amplification signal band after electrophoresis on a 2% agarose gel. Five of 120 samples of clinical serum (4.2%) could not be amplified.
Conclusion:
C-PCR has the advantage of yielding information on false negative in the HBV DNA PCR assay of clinical serum samples.
