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Published on: March 17, 2023
Peroxynitrite activates glucose uptake in 3T3-L1 adipocytes through a PI3-K-dependent mechanism
Alberto M Guzman-Grenfell1, Rebeca Garcia-Macedo, Marco T Gonzalez-Martinez
1Laboratorio de Bioquimica Inorganica, Direccion de Investigacion, Instituto Nacional de Enfermedades Respiratorias, Mexico, DF Mexico. martinguzman@cablevision.net.mx <martinguzman@cablevision.net.mx>
Abstract:
Peroxynitrite, the product of the reaction between *NO and O2*-, is a strong oxidant and nitrating molecule, and it has been recently consideredas a component of some important signaling pathways. Herein, we report the effect of peroxynitrite on glucose uptake in 3T3-L1 adipocytes. Peroxynitrite stimulated glucose uptake and this effect was inhibited by citochalasin B, indicating the participation of facilitated GLUT transporters. Peroxynitrite-induced glucose uptake was not related to intracellular ATP, nor to external or internal calcium, but it was inhibited by the phosphatidylinositol 3-kinase (PI3-K) inhibitor, wortmannin. Additionally, we also found that peroxynitrite did not activate the insulin receptor nor the PI3-K downstream signaling protein kinase B (PKB/Akt). The dose-dependent inhibitory action of wortmannin suggests that peroxynitrite activates glucose transport without affecting GLUT transporters translocation.
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