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Updated: Aug 20, 2026

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
Published on: June 30, 2022
A pre-tRNA carrying intron features typical of Archaea is spliced in yeast
Gianfranco Di Segni1, Lodovica Borghese, Silvia Sebastiani
1Istituto di Biologia Cellulare, CNR, Via E. Ramarini 32, 00016 Monterotondo Scalo (Rome) 00016, Italy.
Abstract:
Archaeal pre-tRNAs are characterized by the presence of the bulge-helix-bulge (BHB) structure in the intron stem-and-loop region. A chimeric pre-tRNA was constructed bearing an intron of the archaeal type and the mature domain of the Saccharomyces cerevisiae suppressor SUP4 tRNA(Tyr). This pre-tRNA(ArchEuka) is correctly cleaved in several cell-free extracts and by purified splicing endonucleases. It is also cleaved and ligated in S. cerevisiae cells, providing efficient suppression of nonsense mutations in various genes.
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