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Published on: December 5, 2016
[Selection of sodium chloride tolerant mutants in Chrysanthemum morifolium in Anhui]
Jian-ping Xue1, Ai-ming Zhang, Xiang Gao
1Department of Biology, Huaibei Coal Industry Teachers' College, Huaibei 235000, China. xuejp2000@yahoo.com.cn
Objective:
To select the sodium chloride tolerant mutants of the Chrysanthemum morifolium callus through tissue culture and EMS (ethylmethane sulfonate) treatment.
Method:
Calli were induced from the leaves of C. morifolium. The calli were treated with 0.2% and 0.5% EMS, respectively. After 15 days' culture, the calli were transplanted to selection media with 0.5%, 1.0% and 1.5% NaCl, and the sodium chloride tolerant mutants were selected out.
Result And Conclusion:
After tested the sodium chloride tolerant stability, the callus selected are found to be the mutants indeed.
