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Related Experiment Video

Updated: Jun 25, 2026

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)
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[Determination of luteolin-7-O-glycoside in the herb of Dracocephalum rupestra by HPLC].

Dong-mei Ren1, Hong-xiang Lou, Bin Ma

  • 1School of Pharmacy, Shandong University, Jinan 250012, China.

Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica
|December 4, 2004
PubMed
Summary
This summary is machine-generated.

This study developed a method to quantify luteolin-7-O-glycoside in Dracocephalum rupestra. The highest concentrations were found in leaves and before the blossoming season.

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Area of Science:

  • Phytochemistry
  • Analytical Chemistry

Context:

  • Dracocephalum rupestra is a medicinal plant with potential therapeutic properties.
  • Luteolin-7-O-glycoside is a key bioactive compound in D. rupestra.

Purpose:

  • To establish a reliable analytical method for quantifying luteolin-7-O-glycoside.
  • To determine the distribution and seasonal variation of luteolin-7-O-glycoside in D. rupestra.

Summary:

  • A simple and accurate method using a Phenomenex C18 column and UV detection at 350 nm was developed.
  • Luteolin-7-O-glycoside content varied across plant parts, with highest levels in leaves and lowest in stems.
  • Seasonal analysis revealed peak concentrations in D. rupestra sampled before blossoming.

Impact:

  • Provides a validated method for quality control of D. rupestra as a herbal medicine.
  • Informs optimal harvesting times and plant parts for maximizing luteolin-7-O-glycoside yield.