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Related Experiment Videos

An accelerated in vitro model for adhesive testing.

William P Kelsey1, Mark A Latta, James D O'Meara

  • 1Creighton University School of Dentistry, 2500 California Plaza, Omaha, NE 68178, USA. wpkelsey@creighton.edu

American Journal of Dentistry
|December 4, 2004
PubMed
Summary

This study developed a lab protocol to test dental adhesive sealing. Results show differences in marginal sealing among tested adhesives after simulated wear and temperature changes.

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Area of Science:

  • Dental Materials Science
  • Restorative Dentistry
  • Biomaterials

Background:

  • Assessing the marginal sealing capability of dental adhesives is crucial for restoration longevity.
  • Existing laboratory testing protocols may not fully simulate clinical challenges.
  • A standardized in vitro protocol is needed for reliable adhesive performance evaluation.

Purpose of the Study:

  • To develop and pilot an accelerated in vitro laboratory testing protocol.
  • To assess the marginal sealing capability of different dental adhesive systems.
  • To evaluate adhesive performance under simulated occlusal wear and thermal stress.

Main Methods:

  • Occlusal cavities in human molars were restored using resin composite and three different adhesive systems.

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  • Specimens underwent sequential challenges: 400,000 wear cycles, 5000 thermocycles, and another 400,000 wear cycles.
  • Microleakage was assessed via silver nitrate staining, and marginal degradation was evaluated using light microscopy and stone casts.

    • Main Results:

    • Median microleakage values were 0 for Prime & Bond NT, 1 for Prompt L-Pop, and 3 for the experimental self-etching adhesive.
    • Significant differences in microleakage were observed between all tested adhesives (P < 0.05).
    • Marginal gaps became more prevalent after the second wear challenge, indicating degradation over time.

    Conclusions:

    • The developed pilot protocol effectively differentiated the marginal sealing capabilities of the tested dental adhesives.
    • Accelerated laboratory testing involving wear and thermocycling can reveal adhesive performance differences.
    • Further validation of this protocol is recommended for broader application in dental materials research.