Related Experiment Video
Updated: Aug 20, 2026
Microhardness Measurements on Tooth and Alveolar Bone in Rodent Oral Disease Models
Published on: April 26, 2024
Monitoring of demineralized dentin microhardness throughout and after bleaching
Patricia Moreira de Freitas1, Cecilia Pedroso Turssi, Anderson Takeo Hara
1Department of Restorative Dentistry, School of Dentistry of Piracicaba, State University of Campinas, Brazil.
Purpose:
To evaluate the effect of six bleaching agents: Nite White [NW] 10% and 22% Excel 2Z (Discus Dental), Rembrandt (REM) 10% and 22% (DenMat), Opalescence (OPA) 10% and 20% (Ultradent) and a placebo agent on demineralized dentin microhardness at different time intervals.
Methods:
105 human dentin slabs (3 x 3 mm) were embedded, planed and submitted to cariogenic challenges, composed of de- and remineralization cycles. For 42 days, specimens were exposed to bleaching agents, consisting of applying them daily for 8 hours, removing and storing the specimens in artificial saliva for 16 hours. At the end of the bleaching treatment, specimens were kept in artificial saliva for 14 days. Knoop Microhardness tests were performed on specimens' surface before (baseline), during (8 hours, 7, 14, 21, 28, 35 and 42 days) and after bleaching procedures (7 and 14 days).
Results:
The ANOVA for split-plot showed significant effect on the interaction between bleaching agent and time (alpha =0.05). Tukey's test revealed no significant differences on demineralized dentin microhardness exposed to bleaching agents until Day 7. Regression analyses demonstrated that NW 10% and 22% and OPA 10% and 20% increased dentin microhardness in different magnitudes, whereas REM 10% and 22% induced mineral loss during bleaching agent application, followed by microhardness recovery in the post-treatment period.
