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Updated: Jul 15, 2026

Expression and Purification of Virus-like Particles for Vaccination
Published on: June 2, 2016
Lentiviral vectors pseudotyped with envelope glycoproteins derived from human parainfluenza virus type 3
Cindy Jung1, Bradley N Grzybowski, Suxiang Tong
1The Wallace H. Coulter Department of Biomedical Engineering at Georgia Tech and Emory University, Atlanta, Georgia 30332-0535, USA.
Abstract:
We describe the generation of lentiviruses pseudotyped with human parainfluenza type 3 envelope (HPIV3) glycoproteins. Lentivirus particles, expressed in 293T/17 cells, incorporate HPIV3 hemagglutinin-neuraminidase (HN) and fusion (F) proteins into their lipid bilayers and are able to transduce human kidney epithelial cells and polarized MDCK cells. Neuraminidase, AZT, and anti-HPIV3 antisera block transduction, which is consistent with lentiviral-mediated transduction via sialated receptors for HPIV3. Our findings show that HPIV3 pseudotyped lentiviruses can be formed and may have a number of useful properties for human gene transfer.
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